%0 Journal Article %J Genes Dev %D 2005 %T Global regulation of X chromosomal genes by the MSL complex in Drosophila melanogaster. %A Hamada, Fumika N %A Park, Peter J %A Gordadze, Polina R %A Kuroda, Mitzi I %K Animals %K Cell Line %K Dosage Compensation, Genetic %K Drosophila melanogaster %K Drosophila Proteins %K Male %K Multiprotein Complexes %K Up-Regulation %K X Chromosome %X

A long-standing model postulates that X-chromosome dosage compensation in Drosophila occurs by twofold up-regulation of the single male X, but previous data cannot exclude an alternative model, in which male autosomes are down-regulated to balance gene expression. To distinguish between the two models, we used RNA interference to deplete Male-Specific Lethal (MSL) complexes from male-like tissue culture cells. We found that expression of many genes from the X chromosome decreased, while expression from the autosomes was largely unchanged. We conclude that the primary role of the MSL complex is to up-regulate the male X chromosome.

%B Genes Dev %V 19 %P 2289-94 %8 2005 Oct 1 %G eng %N 19 %1 http://www.ncbi.nlm.nih.gov/pubmed/16204180?dopt=Abstract %R 10.1101/gad.1343705