@article {564051, title = {MSL complex associates with clusters of actively transcribed genes along the Drosophila male X chromosome.}, journal = {Cold Spring Harb Symp Quant Biol}, volume = {71}, year = {2006}, month = {2006}, pages = {385-94}, abstract = { Dosage compensation in Drosophila serves as a model system for understanding the targeting of chromatin-modifying complexes to their sites of action. The MSL (male-specific lethal) complex up-regulates transcription of the single male X chromosome, thereby equalizing levels of transcription of X-linked genes between the sexes. Recruitment of the MSL complex to its binding sites on the male X chromosome requires each of the MSL proteins and at least one of the two large noncoding roX RNAs. To better understand how the MSL complex specifically targets the X chromosome, we have defined the binding using high-resolution genomic tiling arrays. Our results indicate that the MSL complex largely associates with transcribed genes that are present in clusters along the X chromosome. We hypothesize that after initial recruitment of the MSL complex to the X chromosome by unknown mechanisms, nascent transcripts or chromatin marks associated with active transcription attract the MSL complex to its final targets. Defining MSL-complex-binding sites will provide a tool for understanding functions of large noncoding RNAs that have remained elusive. }, keywords = {Animals, Binding Sites, DNA-Binding Proteins, Dosage Compensation, Genetic, Drosophila melanogaster, Drosophila Proteins, Genes, Insect, Male, Models, Molecular, Multigene Family, Multiprotein Complexes, Nuclear Proteins, Oligonucleotide Array Sequence Analysis, Transcription Factors, Transcription, Genetic, X Chromosome}, issn = {0091-7451}, doi = {10.1101/sqb.2006.71.026}, author = {Larschan, E and Alekseyenko, A A and Lai, W R and Park, P J and Kuroda, M I} }