@article {564016,
	title = {Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.},
	journal = {Blood},
	volume = {114},
	number = {2},
	year = {2009},
	month = {2009 Jul 9},
	pages = {268-78},
	abstract = {
	Surface antigens on hematopoietic stem cells (HSCs) enable prospective isolation and characterization. Here, we compare the cell-surface phenotype of hematopoietic repopulating cells from murine yolk sac, aorta-gonad-mesonephros, placenta, fetal liver, and bone marrow with that of HSCs derived from the in vitro differentiation of murine embryonic stem cells (ESC-HSCs). Whereas c-Kit marks all HSC populations, CD41, CD45, CD34, and CD150 were developmentally regulated: the earliest embryonic HSCs express CD41 and CD34 and lack CD45 and CD150, whereas more mature HSCs lack CD41 and CD34 and express CD45 and CD150. ESC-HSCs express CD41 and CD150, lack CD34, and are heterogeneous for CD45. Finally, although CD48 was absent from all in vivo HSCs examined, ESC-HSCs were heterogeneous for the expression of this molecule. This unique phenotype signifies a developmentally immature population of cells with features of both primitive and mature HSC. The prospective fractionation of ESC-HSCs will facilitate studies of HSC maturation essential for normal functional engraftment in irradiated adults.

},
	keywords = {Animals, Antigens, CD, Cells, Cultured, Embryo, Mammalian, Embryonic Stem Cells, Female, Hematopoietic Stem Cells, Mice, Mice, Inbred C57BL, Phenotype, Placenta},
	issn = {1528-0020},
	doi = {10.1182/blood-2008-12-193888},
	author = {McKinney-Freeman, Shannon L and Naveiras, Olaia and Yates, Frank and Loewer, Sabine and Philitas, Marsha and Curran, Matthew and <b>Park, Peter J</b> and Daley, George Q}
}
