@article {563951, title = {An assessment of histone-modification antibody quality.}, journal = {Nat Struct Mol Biol}, volume = {18}, number = {1}, year = {2011}, month = {2011 Jan}, pages = {91-3}, abstract = { We have tested the specificity and utility of more than 200 antibodies raised against 57 different histone modifications in Drosophila melanogaster, Caenorhabditis elegans and human cells. Although most antibodies performed well, more than 25\% failed specificity tests by dot blot or western blot. Among specific antibodies, more than 20\% failed in chromatin immunoprecipitation experiments. We advise rigorous testing of histone-modification antibodies before use, and we provide a website for posting new test results (http://compbio.med.harvard.edu/antibodies/). }, keywords = {Animals, Antibodies, Antibody Specificity, Blotting, Western, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Chromatin Immunoprecipitation, Drosophila melanogaster, Drosophila Proteins, Histones, Immunoblotting, Protein Processing, Post-Translational, Quality Control, Reproducibility of Results}, issn = {1545-9985}, doi = {10.1038/nsmb.1972}, author = {Egelhofer, Thea A* and Minoda, Aki* and Klugman, Sarit* and Lee, Kyungjoon and Kolasinska-Zwierz, Paulina and Alekseyenko, Artyom A and Cheung, Ming-Sin and Day, Daniel S and Gadel, Sarah and Gorchakov, Andrey A and Gu, Tingting and Kharchenko, Peter V and Kuan, Samantha and Latorre, Isabel and Linder-Basso, Daniela and Luu, Ying and Ngo, Queminh and Perry, Marc and Rechtsteiner, Andreas and Riddle, Nicole C and Schwartz, Yuri B and Shanower, Gregory A and Vielle, Anne and Ahringer, Julie and Elgin, Sarah C R and Kuroda, Mitzi I and Pirrotta, Vincenzo and Ren, Bing and Strome, Susan and Park, Peter J** and Karpen, Gary H** and Hawkins, R David** and Lieb, Jason D**} }