@article {1611161,
	title = {Essential histone chaperones collaborate to regulate transcription and chromatin integrity},
	journal = {Genes Dev},
	volume = {35},
	number = {9-10},
	year = {2021},
	month = {2021 05 01},
	pages = {698-712},
	abstract = {Histone chaperones are critical for controlling chromatin integrity during transcription, DNA replication, and DNA repair. Three conserved and essential chaperones, Spt6, Spn1/Iws1, and FACT, associate with elongating RNA polymerase II and interact with each other physically and/or functionally; however, there is little understanding of their individual functions or their relationships with each other. In this study, we selected for suppressors of a temperature-sensitive spt6 mutation that disrupts the Spt6-Spn1 physical interaction and that also causes both transcription and chromatin defects. This selection identified novel mutations in FACT. Surprisingly, suppression by FACT did not restore the Spt6-Spn1 interaction, based on coimmunoprecipitation, ChIP, and mass spectrometry experiments. Furthermore, suppression by FACT bypassed the complete loss of Spn1. Interestingly, the FACT suppressor mutations cluster along the FACT-nucleosome interface, suggesting that they alter FACT-nucleosome interactions. In agreement with this observation, we showed that the spt6 mutation that disrupts the Spt6-Spn1 interaction caused an elevated level of FACT association with chromatin, while the FACT suppressors reduced the level of FACT-chromatin association, thereby restoring a normal Spt6-FACT balance on chromatin. Taken together, these studies reveal previously unknown regulation between histone chaperones that is critical for their essential in vivo functions.},
	issn = {1549-5477},
	doi = {10.1101/gad.348431.121},
	author = {Viktorovskaya, Olga and Chuang, James and <b>Jain, Dhawal</b> and Reim, Natalia I and L{\'o}pez-Rivera, Francheska and Murawska, Magdalena and Spatt, Dan and Churchman, L Stirling and <b>Park, Peter J</b> and Winston, Fred}
}
