@article {152821, title = {Swi/Snf chromatin remodeling/tumor suppressor complex establishes nucleosome occupancy at target promoters.}, journal = {Proc Natl Acad Sci U S A}, volume = {110}, number = {25}, year = {2013}, month = {2013 Jun 18}, pages = {10165-70}, abstract = { Precise nucleosome-positioning patterns at promoters are thought to be crucial for faithful transcriptional regulation. However, the mechanisms by which these patterns are established, are dynamically maintained, and subsequently contribute to transcriptional control are poorly understood. The switch/sucrose non-fermentable chromatin remodeling complex, also known as the Brg1 associated factors complex, is a master developmental regulator and tumor suppressor capable of mobilizing nucleosomes in biochemical assays. However, its role in establishing the nucleosome landscape in vivo is unclear. Here we have inactivated Snf5 and Brg1, core subunits of the mammalian Swi/Snf complex, to evaluate their effects on chromatin structure and transcription levels genomewide. We find that inactivation of either subunit leads to disruptions of specific nucleosome patterning combined with a loss of overall nucleosome occupancy at a large number of promoters, regardless of their association with CpG islands. These rearrangements are accompanied by gene expression changes that promote cell proliferation. Collectively, these findings define a direct relationship between chromatin-remodeling complexes, chromatin structure, and transcriptional regulation. }, keywords = {Animals, Cell Proliferation, Chromatin, Chromosomal Proteins, Non-Histone, CpG Islands, DNA Helicases, Fibroblasts, Gene Expression Regulation, Neoplastic, Gene Knockdown Techniques, Mice, Neoplasms, Nuclear Proteins, Nucleosomes, Primary Cell Culture, Promoter Regions, Genetic, Protein Binding, Transcription Factors, Transcriptional Activation}, issn = {1091-6490}, doi = {10.1073/pnas.1302209110}, author = {Tolstorukov, Michael Y* and Sansam, Courtney G* and Lu, Ping* and Koellhoffer, Edward C and Helming, Katherine C and Alver, Burak Han and Tillman, Erik J and Evans, Julia A and Wilson, Boris G and Park, Peter J** and Roberts, Charles W M**} }