@article {1068426,
	title = {Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation},
	journal = {Mol Cell},
	volume = {66},
	number = {1},
	year = {2017},
	month = {2017 Apr 06},
	pages = {77-88.e5},
	abstract = {
	Spt5 is an essential and conserved factor that functions in transcription and co-transcriptional processes. However, many aspects of the requirement for Spt5 in transcription are poorly understood. We\&nbsp;have analyzed the consequences of Spt5 depletion in Schizosaccharomyces pombe using four genome-wide approaches. Our results demonstrate that Spt5 is crucial for a normal rate of RNA synthesis and distribution of RNAPII over transcription units. In\&nbsp;the absence of Spt5, RNAPII localization changes dramatically, with reduced levels and a relative accumulation over the first \~{}500\&nbsp;bp, suggesting that Spt5 is required for transcription past a barrier. Spt5 depletion also results in widespread antisense transcription initiating within this barrier region. Deletions of this region alter the distribution of RNAPII on\&nbsp;the sense strand, suggesting that the barrier observed after Spt5 depletion is normally a site at\&nbsp;which Spt5 stimulates elongation. Our results reveal a global requirement for Spt5 in transcription elongation.

},
	issn = {1097-4164},
	doi = {10.1016/j.molcel.2017.02.023},
	author = {Shetty, Ameet* and Kallgren, Scott P* and Demel, Carina and Maier, Kerstin C and Spatt, Dan and Alver, Burak H and Cramer, Patrick and <b>Park, Peter J</b> and Winston, Fred}
}
